TitleAn analytical investigation of 24 oxygenated-PAHs (OPAHs) using liquid and gas chromatography-mass spectrometry.
Publication TypeJournal Article
Year of Publication2013
AuthorsO'Connell, SG, Haigh, T, Wilson, G, Anderson, KA
JournalAnal Bioanal Chem
Volume405
Issue27
Pagination8885-96
Date Published2013 Nov
ISSN1618-2650
KeywordsAtmospheric Pressure, Calibration, Chromatography, Liquid, Environmental Pollutants, Gas Chromatography-Mass Spectrometry, Observer Variation, Polycyclic Aromatic Hydrocarbons, Reference Standards, Reproducibility of Results, Sensitivity and Specificity, Solutions
Abstract

We developed two independent approaches for separation and quantitation of 24 oxygenated polycyclic aromatic hydrocarbons (OPAHs) using both liquid chromatography-atmospheric pressure chemical ionization/mass spectrometry (LC-APCI/MS) and gas chromatography-electron impact/mass spectrometry (GC-EI/MS). Building on previous OPAH research, we examined laboratory stability of OPAHs, improved existing method parameters, and compared quantification strategies using standard addition and an internal standard on an environmental sample. Of 24 OPAHs targeted in this research, 19 compounds are shared between methods, with 3 uniquely quantitated by GC-EI/MS and 2 by LC-APCI/MS. Using calibration standards, all GC-EI/MS OPAHs were within 15 % of the true value and had less than 15 % relative standard deviations (RSDs) for interday variability. Similarly, all LC-APCI/MS OPAHs were within 20 % of the true value and had less than 15 % RSDs for interday variability. Instrument limits of detection ranged from 0.18 to 36 ng mL(-1) on the GC-EI/MS and 2.6 to 26 ng mL(-1) on the LC-APCI/MS. Four standard reference materials were analyzed with each method, and we report some compounds not previously published in these materials, such as perinaphthenone and xanthone. Finally, an environmental passive sampling extract from Portland Harbor Superfund, OR was analyzed by each method using both internal standard and standard addition to compensate for potential matrix effects. Internal standard quantitation resulted in increased precision with similar accuracy to standard addition for most OPAHs using 2-fluoro-fluorenone-(13)C as an internal standard. Overall, this work improves upon OPAH analytical methods and provides some considerations and strategies for OPAHs as focus continues to expand on this emerging chemical class.

DOI10.1007/s00216-013-7319-x
Alternate JournalAnal Bioanal Chem
PubMed ID24005604
PubMed Central IDPMC3824841
Grant ListR21 ES020120 / ES / NIEHS NIH HHS / United States
T32ES007060-32 / ES / NIEHS NIH HHS / United States
P42 ES016465 / ES / NIEHS NIH HHS / United States
T32 ES007060 / ES / NIEHS NIH HHS / United States
P30 ES000210 / ES / NIEHS NIH HHS / United States