TitleOptimizing multi-dimensional high throughput screening using zebrafish.
Publication TypeJournal Article
Year of Publication2016
AuthorsTruong, L, Bugel, SM, Chlebowski, A, Usenko, CY, Simonich, MT, Simonich, SLMassey, Tanguay, RL
JournalReprod Toxicol
Volume65
Pagination139-147
Date Published2016 10
ISSN1873-1708
KeywordsAnimals, Embryo, Nonmammalian, Estradiol, Ethinyl Estradiol, High-Throughput Screening Assays, Thiram, Zebrafish
Abstract

The use of zebrafish for high throughput screening (HTS) for chemical bioactivity assessments is becoming routine in the fields of drug discovery and toxicology. Here we report current recommendations from our experiences in zebrafish HTS. We compared the effects of different high throughput chemical delivery methods on nominal water concentration, chemical sorption to multi-well polystyrene plates, transcription responses, and resulting whole animal responses. We demonstrate that digital dispensing consistently yields higher data quality and reproducibility compared to standard plastic tip-based liquid handling. Additionally, we illustrate the challenges in using this sensitive model for chemical assessment when test chemicals have trace impurities. Adaptation of these better practices for zebrafish HTS should increase reproducibility across laboratories.

DOI10.1016/j.reprotox.2016.05.015
Alternate JournalReprod. Toxicol.
PubMed ID27453428
PubMed Central IDPMC5067206
Grant ListF31 ES026037 / ES / NIEHS NIH HHS / United States
K99 ES025280 / ES / NIEHS NIH HHS / United States
P42 ES016465 / ES / NIEHS NIH HHS / United States
T32 ES007060 / ES / NIEHS NIH HHS / United States
P30 ES000210 / ES / NIEHS NIH HHS / United States