TitleVitamin E is necessary for zebrafish nervous system development.
Publication TypeJournal Article
Year of Publication2020
AuthorsHead, B, La Du, J, Tanguay, RL, Kioussi, C, Traber, MG
JournalSci Rep
Volume10
Issue1
Pagination15028
Date Published2020 09 21
ISSN2045-2322
KeywordsAnimals, Brain, Carrier Proteins, Embryo, Nonmammalian, Gastrulation, Gene Expression Regulation, Developmental, Nervous System, PAX2 Transcription Factor, SOXE Transcription Factors, Vitamin E, Vitamin E Deficiency, Zebrafish, Zebrafish Proteins
Abstract

Vitamin E (VitE) deficiency results in embryonic lethality. Knockdown of the gene ttpa encoding for the VitE regulatory protein [α-tocopherol transfer protein (α-TTP)] in zebrafish embryos causes death within 24 h post-fertilization (hpf). To test the hypothesis that VitE, not just α-TTP, is necessary for nervous system development, adult 5D strain zebrafish, fed either VitE sufficient (E+) or deficient (E-) diets, were spawned to obtain E+ and E- embryos, which were subjected to RNA in situ hybridization and RT-qPCR. Ttpa was expressed ubiquitously in embryos up to 12 hpf. Early gastrulation (6 hpf) assessed by goosecoid expression was unaffected by VitE status. By 24 hpf, embryos expressed ttpa in brain ventricle borders, which showed abnormal closure in E- embryos. They also displayed disrupted patterns of paired box 2a (pax2a) and SRY-box transcription factor 10 (sox10) expression in the midbrain-hindbrain boundary, spinal cord and dorsal root ganglia. In E- embryos, the collagen sheath notochord markers (col2a1a and col9a2) appeared bent. Severe developmental errors in E- embryos were characterized by improper nervous system patterning of the usually carefully programmed transcriptional signals. Histological analysis also showed developmental defects in the formation of the fore-, mid- and hindbrain and somites of E- embryos at 24 hpf. Ttpa expression profile was not altered by the VitE status demonstrating that VitE itself, and not ttpa, is required for development of the brain and peripheral nervous system in this vertebrate embryo model.

DOI10.1038/s41598-020-71760-x
Alternate JournalSci Rep
PubMed ID32958954
PubMed Central IDPMC7506018
Grant ListP30 ES030287 / ES / NIEHS NIH HHS / United States